Recombinant human ST6GAL1, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
ST6GAL1, also known as beta-galactoside alpha-2,6-sialyltransferase 1, is a type II membrane protein localized in the trans-Golgi network and catalyzes 2,6-sialylation of Gal beta 1,4-GlcNAc structures on N-glycans. It is highly expressed in the liver and also expressed in most other tissues to some extent. Its deficiency causes abnormalities in B cell immunoreactivity.
Liquid. In Phosphate Buffered Saline (pH 7.4) containing 10% glycerol.
44.6kDa (389aa)40-57kDa (SDS-PAGE under reducing conditions)
ADPKEKKKGS YYDSFKLQTK EFQVLKSLGK LAMGSDSQSV SSSSTQDPHR GRQTLGSLRG LAKAKPEASF QVWNKDSSSK NLIPRLQKIWKNYLSMNKYK VSYKGPGPGI KFSAEALRCH LRDHVNVSMV EVTDFPFNTS EWEGYLPKES IRTKAGPWGR CAVVSSAGSL KSSQLGREIDDHDAVLRFNG APTANFQQDV GTKTTIRLMN SQLVTTEKRF LKDSLYNEGI LIVWDPSVYH SDIPKWYQNP DYNFFNNYKT YRKLHPNQPFYILKPQMPWE LWDILQEISP EEIQPNPPSS GMLGIIIMMT LCDQVDIYEF LPSKRKTDVC YYYQKFFDSA CTMGAYHPLL YEKNLVKHLNQGTDEDIYLL GKATLPGFRT IHCHHHHHH
< 1.0 EU per 1 microgram of protein (determined by LAL method)
>90% by SDS - PAGE
Can be stored at +4centigrade short term (1-2 weeks). For long term storage, aliquot and store at -20Centigrade or -70Centigrade. Avoid repeated freezing and thawing cycles.
0.5mg/ml (determined by Absorbance at 280nm)