Recombinant Human STK38L(1 a.a. - 464 a.a.) fused with GST tag was expressed in Insect cells.
STK38L played an important role in many functions.
In 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.05% Brij35, 1 mM DTT, 10% glycerol)
The activity was measured by off-chip mobility shift assay. The enzyme was incubated with fluorescence-labeled substrate and Mg(or Mn)/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by LabChip 3000. Substrate: SGKtide. ATP: 100 uM.
72 % by SDS-PAGE/CBB staining
Functional Study; SDS-PAGE
Store at -80 centigrade.Aliquot to avoid repeated freezing and thawing.