Recombinant human Cripto-1 cDNA (31-150aa, isoform-1, derived from BC067844) fused with T7-His-TEV cleavage site Tag (29aa) at N-terminal was expressed in E. coli.
0.2 mg/ml, sterile-filtered, in 20 mM pH 8.0 Tris-HCl Buffer, with proprietary formulation of NaCl, KCl, EDTA, arginine, DTT and Glycerol.
Measured by its binding ability in a functional ELSA. Immobilized recombinant human Nodal protein at 2ug/ml (100ul/well) can bind rhCripto-1 with a linear range of 0.5-100 ng/ml.
1. May be used for in vitro Cripto-1/Nodal signaling pathway regulations study for mesoderm differentiation.2. Potential biomarker protein for various cancer diagnostic developments.3. May be used as antigen for specific antibody production.
Keep at -80centigrade for long term storage. Product is stable at 4 centigrade for at least 30 days.