Recombinant Human TIE2 (R915C) (771-end) fused with GST tag at N-terminal was expressed in Sf9 insect cells.
TIE2 or TEK is a receptor tyrosine kinase that is expressed principally on vascular endothelium. Disrupting TIE2 function in mice results in embryonic lethality with defects in embryonic vasculature, suggests a role in blood vessel maturation and maintenance. Angiopoietin-1 is a secreted growth factor that binds to and activates the TIE2 receptor tyrosine kinase. SHP2 and GRB2 are recruited to the activated TIE 2 kinase domain and are part of the cellular responses that mediate TIE2 function. TIE2 expression is upregulated in the endothelium of vascular "hot spots" in human breast cancer specimens. However, TIE2 is also overexpressed in areas of active angiogenesis in normal tissues.
50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
The specific activity was determined to be 100 nmol/min/mg.
>85% by densitometry
Store product at –70 centigrade. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.