Recombinant human TIMP2, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
TIMP2, also known as metalloproteinase inhibitor 2, belongs to the tissue inhibitors of metalloproteinases family. The TIMP family inhibit most MMPs by forming non-covalent binary complex. TIMP2 plays a unique role among TIMP family members owing to its functions to regulate cellular responses to growth factors.
Liquid. In Phosphate Buffered Saline (pH 7.4) containing 10% glycerol.
22.5kDa (200aa)18-28KDa (SDS–PAGE under reducing conditions.)
CSCSPVHPQQ AFCNADVVIR AKAVSEKEVD SGNDIYGNPI KRIQYEIKQI KMFKGPEKDI EFIYTAPSSA VCGVSLDVGG KKEYLIAGKAEGDGKMHITL CDFIVPWDTL STTQKKSLNH RYQMGCECKI TRCPMIPCYI SSPDECLWMD WVTEKNINGH QAKFFACIKR SDGSCAWYRGAAPPKQEFLD IEDPHHHHHH
< 1.0 EU per 1 microgram of protein (determined by LAL method)
>95% by SDS – PAGE
Can be stored at +4 centigrade short term (1-2 weeks). For long term storage, aliquot and store at -20 centigrade or -70 centigrade. Avoid repeated freezing and thawing cycles.
0.25mg/ml (determined by Absorbance at 280nm)