Recombinant N-terminal fragment of human TLE3 (Q04726-1) (Ser 484-Tyr 772) was fused with the GST tag at the N-terminus.
Predicted N Terminal:
Lyophilized from sterile 20mM Tris, 0.15M NaCl, 5mM GSH, pH 7.5, 5%~8% trehalose and mannitol.
The recombinant human TLE3/GST chimera consists of 520 amino acids and has a predicted molecular mass of 58.5 kDa as estimated in SDS-PAGE under reducing conditions.
< 1.0 EU per μg of the protein as determined by the LAL method.
>78 % as determined by SDS-PAGE.
Samples are stable for up to twelve months from date of receipt at -70ºC.
Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.