Recombinant Human TLR3(Lys27-Ser711) fused with His tag at C-terminal was expressed in NS0.
Predicted N Terminal:
Lyophilized from a 0.2 μm filtered solution in PBS.
Measured by its ability to inhibit poly I:C induced IL-8 secretion by HEK293 human embryonic kidney cells transfected with TLR3. The ED50 for this effect is typically 5-10 µg/mL in the presence of 10 µg/mL of poly I:C.
Predicted Molecular Mass: 79.2 kDa;SDS-PAGE: 110-120 kDa, reducing conditions.
<0.1 EU per 1 mug of the protein by the LAL method.
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70 centigrade as supplied.1 month, 2 to 8 centigrade under sterile conditions after reconstitution.3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitute at 400 μg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.