Recombinant Human TNF Protein, His-tagged

Cat.No.: TNF-3315H
Product Overview: Human TNF-alpha Protein, His Tag, Val 77 - Leu 233 (Accession # NP_000585.2) was produced in human 293 cells (HEK293).
Source: HEK293
Species: Human
Tag: His
Predicted N Terminal: Val 77
Form: Lyophilized from sterile PBS, pH7.4, 10% Trehalose.
Bio-activity: Measured by its binding ability in a functional ELISA. Immobilized Human TNF-alpha, His Tag at 0.2 μg/mL (100 μl/well) can bind Human TNFR1, Fc Tag with a linear range of 1.5-25 ng/mL.
Molecular Mass: Human TNF-alpha, His Tag is fused with a polyhistidine tag at the C-terminus, and has a calculated MW of 18.2 kDa. The reducing protein migrates as 18.2 kDa in SDS-PAGE.
Endotoxin: Less than 1.0 EU per μg by the LAL method.
Purity: >95% as determined by SDS-PAGE.
Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
Storage: Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.
Gene Name: TNF tumor necrosis factor [ Homo sapiens ]
Official Symbol: TNF
Synonyms: TNF; tumor necrosis factor; TNFA, tumor necrosis factor (TNF superfamily, member 2); DIF; TNF superfamily; member 2; TNF alpha; TNFSF2; TNF-a; cachectin; APC1 protein; TNF, monocyte-derived; TNF, macrophage-derived; TNF superfamily, member 2; tumor necrosis factor alpha; tumor necrosis factor-alpha; tumor necrosis factor ligand superfamily member 2; TNFA; TNF-alpha;
Gene ID: 7124
mRNA Refseq: NM_000594
Protein Refseq: NP_000585
MIM: 191160
UniProt ID: P01375
Chromosome Location: 6p21.3
Pathway: Adipocytokine signaling pathway, organism-specific biosystem; Adipocytokine signaling pathway, conserved biosystem; Adipogenesis, organism-specific biosystem; African trypanosomiasis, organism-specific biosystem; African trypanosomiasis, conserved biosystem; Allograft rejection, organism-specific biosystem; Allograft rejection, conserved biosystem;
Function: cytokine activity; identical protein binding; protease binding; protein binding; transcription regulatory region DNA binding; tumor necrosis factor receptor binding;

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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