Human TNF-alpha Protein, His Tag, Val 77 - Leu 233 (Accession # NP_000585.2) was produced in human 293 cells (HEK293).
Predicted N Terminal:
Lyophilized from sterile PBS, pH7.4, 10% Trehalose.
Measured by its binding ability in a functional ELISA. Immobilized Human TNF-alpha, His Tag at 0.2 μg/mL (100 μl/well) can bind Human TNFR1, Fc Tag with a linear range of 1.5-25 ng/mL.
Human TNF-alpha, His Tag is fused with a polyhistidine tag at the C-terminus, and has a calculated MW of 18.2 kDa. The reducing protein migrates as 18.2 kDa in SDS-PAGE.
Less than 1.0 EU per μg by the LAL method.
>95% as determined by SDS-PAGE.
Samples are stable for up to twelve months from date of receipt at -70ºC.
Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.