Recombinant human TNFRSF1A, fused to hIgG-His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
TNFRSF1A, also known as tumor necrosis factor receptor superfamily member 1A, is a member of CD family, tumor necrosis factor receptor superfamily. It is one of the most primary receptors for the tumor necrosis factor-alpha. It has been shown to be localized to both plasma membrane lipid rafts and the trans golgi complex with the help of the death domain.
Liquid. In Phosphate Buffered Saline (pH 7.4) containing 10% glycerol.
47.7kDa (424aa)57-70KDa (SDS–PAGE under reducing conditions.)
ADPLVPHLGD REKRDSVCPQ GKYIHPQNNS ICCTKCHKGT YLYNDCPGPG QDTDCRECES GSFTASENHL RHCLSCSKCR KEMGQVEISSCTVDRDTVCG CRKNQYRHYW SENLFQCFNC SLCLNGTVHL SCQEKQNTVC TCHAGFFLRE NECVSCSNCK KSLECTKLCL PQIENVKGTEDSGTTLEPKS CDKTHTCPPC PAPELLGGPS VFLFPPKPKD TLMISRTPEV TCVVVDVSHE DPEVKFNWYV DGVEVHNAKT KPREEQYNSTYRVVSVLTVL HQDWLNGKEY KCKVSNKALP APIEKTISKA KGQPREPQVY TLPPSRDELT KNQVSLTCLV KGFYPSDIAV EWESNGQPENNYKTTPPVLD SDGSFFLYSK LTVDKSRWQQ GNVFSCSVMH EALHNHYTQK SLSLSPGKHH HHHH
< 1.0 EU per 1 microgram of protein (determined by LAL method)
>90% by SDS - PAGE
Can be stored at +4centigrade short term (1-2 weeks). For long term storage, aliquot and store at -20Centigrade or -70Centigrade. Avoid repeated freezing and thawing cycles.
0.5mg/ml (determined by Absorbance at 280nm)