Active Recombinant Human TNFRSF4 protein, His & Avi-tagged, Biotinylated

• Cat.No.: TNFRSF4-1826H
• Product Overview: Biotinylated Human OX40 / TNFRSF4 / CD134, His Tag is expressed from human 293 cells (HEK293). It contains AA Leu 29 - Ala 216 (Accession # NP_003318.1). Biotinylation of this product is performed using Avitag technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin. The biotin to protein ratio is 0.5-1 as determined by the HABA assay.

Specification

• Description: Tumor necrosis factor receptor superfamily member 4 (TNFRSF4) is also known as ACT35 antigen, OX40L receptor, TAX transcriptionally-activated glycoprotein 1 receptor, CD antigen CD134, OX40. OX40 / TNFRSF4 contains four TNFR-Cys repeats. TNFRSF4 is receptor for TNFSF4 / OX40L / GP34 and can interacts with TRAF2, TRAF3 and TRAF5.
• Source: HEK293
• Species: Human
• Tag: His & Avi
• Predicted N Terminal: Leu 29
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4, 10% trehalose.
• Bio-activity: Immobilized Anti-OX40 MAb, Human IgG1 at 2 μg/mL (100 μL/well) can bind Biotinylated Human OX40, His Tag with a linear range of 1-5 ng/mL.
• Molecular Mass: This protein carries an Avi tag at the C-terminus, followed by a polyhistidine tag. The protein has a calculated MW of 22.8 kDa. The protein migrates as 40-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Protein length: AA Leu 29 - Ala 216
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. No activity loss is observed after storage at: 4-8°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: TNFRSF4 TNF receptor superfamily member 4 [ Homo sapiens (human) ]
• Official Symbol: TNFRSF4
• Synonyms: OX40; ACT35; CD134; IMD16; TXGP1L; ACT35 antigen; ATC35 antigen; CD134 antigen; OX40 antigen; OX40 cell surface antigen; OX40 homologue; OX40L receptor; TAX transcriptionally-activated glycoprotein 1 receptor; lymphoid activation antigene ACT35; tax-transcriptionally activated glycoprotein 1 receptor
• Gene ID: 7293
• mRNA Refseq: NM_003327.3
• Protein Refseq: NP_003318.1
• UniProt ID: P43489