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Active Recombinant Human TNFRSF9 protein, Fc-tagged

Cat.No. : TNFRSF9-232H
Product Overview : Recombinant Human TNFRSF9(Leu24-Gln186) fused with Fc region of Human IgG1 at C-terminal was expressed in HEK293.
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Description : 4-1BB, also known as CD137 and TNFRSF9, is an approximately 30 kDa transmembrane glycoprotein in the TNF receptor superfamily. 4-1BB functions in the development and activation of multiple immune cells. Mature human 4-1BB consists of a 163 amino acid (aa) extracellular domain (ECD) with four TNFR cysteine‑rich repeats, a 27 aa transmembrane segment, and a 42 aa cytoplasmic domain. Within the ECD, cynomolgus 4-1BB shares 95%, 57%, and 57% aa sequence identity with human, mouse, and rat 4-1BB, respectively. 4-1BB is expressed as a disulfide-linked homodimer on various populations of activated T cell including CD4+, CD8+, memory CD8+, NKT, and regulatory T cells as well as on myeloid and mast cell progenitors, dendritic cells, mast cells, and bacterially infected osteoblasts. It binds with high affinity to the transmembrane 4-1BB Ligand/TNFSF9 which is expressed on antigen presenting cells and myeloid progenitor cells. This interaction co-stimulates the proliferation, activation, and/or survival of the 4-1BB expressing cell. It can also enhance the activation-induced cell death of repetitively stimulated T cells. Mice lacking 4-1BB show augmented T cell activation, perhaps due to its absence on regulatory T cells.
4-1BB can associate with OX40 on activated T cells, forming a complex that responds to either ligand and inhibits Treg and CD8+ T cell proliferation. Reverse signaling through 4-1BB Ligand inhibits the development of dendritic cells, B cells, and osteoclasts but supports mature dendritic cell survival and co‑stimulates the proliferation and activation of mast cells. 4-1BB activation enhances CD8+ T cell and NK cell mediated anti-tumor immunity. It also contributes to the development of inflammation in high fat diet-induced metabolic syndrome. Soluble forms of 4-1BB and 4-1BB Ligand circulate at elevated levels in the serum of rheumatoid arthritis and hematologic cancer patients, respectively.
Source : HEK293
Species : Human
Tag : Fc
Predicted N Terminal : Leu24
Form : Lyophilized from a 0.2 μm filtered solution in PBS.
Bio-activity : Measured by its binding ability in a functional ELISA. When Recombinant Cynomolgus Monkey 4-1BB/TNFRSF9/CD137 Fc Chimera is immobilized at 0.05 µg/mL (100 µL/well), Recombinant Human 4-1BB Ligand/TNFSF9 binds with an ED50 of 0.25-1.25 ng/mL.
Molecular Mass : Predicted Molecular Mass: 44 kDa
SDS-PAGE: 56-66 kDa, reducing conditions
Endotoxin : <0.1 EU per 1 μg of the protein by the LAL method.
Purity : >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Storage : Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution : Reconstitute at 500 μg/mL in PBS.
Gene Name : TNFRSF9 tumor necrosis factor receptor superfamily, member 9 [ Homo sapiens ]
Official Symbol : TNFRSF9
Synonyms : TNFRSF9; tumor necrosis factor receptor superfamily, member 9; ILA; tumor necrosis factor receptor superfamily member 9; 4 1BB; CD137; CD137 antigen; T cell antigen ILA; T-cell antigen ILA; 4-1BB ligand receptor; homolog of mouse 4-1BB; receptor protein 4-1BB; T-cell antigen 4-1BB homolog; induced by lymphocyte activation (ILA); interleukin-activated receptor, homolog of mouse Ly63; 4-1BB; CDw137; MGC2172; FLJ43501;
Gene ID : 3604
mRNA Refseq : NM_001561
Protein Refseq : NP_001552
MIM : 602250
UniProt ID : Q07011

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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What diseases are associated with abnormal expression levels of TNFRSF9? 12/10/2022

Studies have found that abnormal expression levels of TNFRSF9 may be associated with certain tumors, autoimmune diseases, and inflammatory diseases.

What is the association of TNFRSF9 with other cytokines? 10/27/2022

It has a variety of associations with other proteins and cytokines. For example, it may interact with the NF-kB signaling pathway to regulate the inflammatory response, and it may also interact with apoptosis-related proteins to regulate the apoptosis process.

What are the main functions of TNFRSF9 in the human body? 07/11/2022

TNFRSF9 mainly plays an important role in the immune system, as an activating signaling molecule, it is able to stimulate the activation, proliferation and survival of T cells and NK cells.

Are there potential therapeutic applications for TNFRSF9 inhibitors or activators? 08/21/2021

Inhibitors or activators targeting TNFRSF9 may have potential applications for the treatment of certain tumors, autoimmune diseases, and inflammatory diseases.

How does TNFRSF9 bind to its ligands? 06/08/2021

The extracellular domain of TNFRSF9 can bind to its ligand, such as 4-1BBL, to initiate signal transduction, which in turn activates downstream signaling pathways.

How is TNFRSF9 related to immuno-oncology? 05/27/2020

TNFRSF9 has some potential in tumor immunotherapy. Several antibody drugs have been developed to use the stimulating effects of TNFRSF9 to enhance the activity of immune cells to fight tumors.

Customer Reviews (3)

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Reviews
03/11/2022

    Good heat resistance, long time treatment at high temperature, still has activity.

    10/11/2021

      The results obtained by SDS-PAGE or Western blot analysis were good.

      07/30/2021

        The purity is good, and the experimental results obtained are accurate.

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