Recombinant Human TNFSF18(Gln 50 - Ser 177) fused with a human IgG1 Fc tag at the N-terminus was expressed in HEK293.
Predicted N Terminal:
Lyophilized from 0.22 μm filtered solution in 50 mM tris, 100 mM glycine, pH7.5. Normally Mannitol or Trehalose are added as protectants before lyophilization.
Has a calculated MW of 46.2 kDa. As a result of glycosylation, the reducing (R) protein migrates as 46 kDa and non-reducing protein migrates as 90 kDa in SDS-PAGE
Less than 1.0 EU per μg of the rh GITR Ligand / TNFSF18 Fc Chimera by the LAL method.
>95% as determined by SDS-PAGE.
Avoid repeated freeze-thaw cycles.No activity loss was observed after storage at:In lyophilized state for 1 year (4 centigrade); After reconstitution under sterile conditions for 3 months (-70 centigrade).