Recombinant Human TNNI3 was expressed in E. coli.
Troponin I (TnI), along with troponin T (TnT) and troponin C (TnC), is one of 3 subunits that form the troponin complex of the thin filaments of striated muscle. TnI is the inhibitory subunit; blocking actin-myosin interactions and thereby mediating striated muscle relaxation. The TnI subfamily contains three genes: TnI-skeletal-fast-twitch, TnI-skeletal-slow-twitch, and TnI-cardiac. This gene encodes the TnI-cardiac protein and is exclusively expressed in cardiac muscle tissues.
6 M Urea, 6 M Tris, pH 8.
210 amino acids
Greater than 98% by SDS-PAGE and HPLC
Store lyophilized at 4 centigrade. Once reconstituted, aliquot and store at -20 centigrade. Avoid freeze-thaw cycles.