Address: 45-1 Ramsey Road, Shirley, NY 11967, USA    USA: 1-631-559-9269  1-631-448-7888   Fax: 1-631-938-8127  Europe: 44-207-048-3343   Email: info@creative-biomart.com
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Recombinant Human UBB protein

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His(6)
N/A(2)
Cat.No.:
UBB-572H
Product Overview:
Recombinant Human UBB was expressed in bacterial.
Source:
E. coli
Species:
Human
Form:
X mg/ml (X μM) in 50 mM HEPES pH 7.5, 200 mM NaCl
Bio-activity:
Di-Ub (K48-linked) FRET TAMRA Pos1 is ideal for use as a substrate for Ubiquitin-specific hydrolases with activity toward K48 linkages. We recommend an assay buffer consisting of 50 mM Hepes pH 7.5, 150 mM NaCl, 2 mM DTT. Fluorescence can be monitored with an excitation wavelength of 544 nM and an emission wavelength of 572 nM. Reaction conditions will need to be optimized for each specific application. We recommend an initial Di-Ub (K48-linked) FRET TAMRA Pos1 concentration of 100-500 nM.
Molecular Mass:
Predicted Molecular Mass: 17 kDa
Purity:
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain
Storage:
Protect from light and avoid repeated freeze-thaw cycles.12 months from date of receipt, -70 centigrade as supplied.3 months, -70 centigrade under sterile conditions after opening.


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Note: There will be extra charge for optional service!
Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127
EMAIL: info@creative-biomart.com

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