Active Recombinant Human VCAM1 protein, His-tagged

• Cat.No.: VCAM1-170H
• Product Overview: Recombinant Human VCAM1(Phe25-Glu698) fused with His tag at the C-terminus was expressed in NS0.

Specification

• Source: Mammalian cells
• Species: Human
• Tag: His
• Predicted N Terminal: Met
• Form: Lyophilized from a 0.2 μm filtered solution in PBS.
• Bio-activity: Measured by the ability of the immobilized protein to support the adhesion of U937 human histiocytic lymphoma cells. When 5 x 10^4 cells/well are added to human VCAM-1 coated plates (10 µg/mL with 100 µL/well), approximately 90-100% will adhere after 1 hour incubation at RT.Optimal dilutions should be determined by each laboratory for each application.
• Molecular Mass: Predicted Molecular Mass: 101.6 (monomer) kDa;SDS-PAGE: 130-140 kDa, reducing conditions
• Endotoxin: <0.10 EU per 1 μg of the protein by the LAL method.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Storage: Avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70 centigrade as supplied.1 month, 2 to 8 centigrade under sterile conditions after reconstitution.3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
• Reconstitution: Reconstitute at 100 μg/mL in sterile PBS.

Gene Information

• Gene Name: VCAM1 vascular cell adhesion molecule 1 [ Homo sapiens ]
• Official Symbol: VCAM1
• Synonyms: VCAM1; vascular cell adhesion molecule 1; vascular cell adhesion protein 1; CD106; CD106 antigen; INCAM-100; MGC99561; DKFZp779G2333
• Gene ID: 7412
• mRNA Refseq: NM_001078
• Protein Refseq: NP_001069
• MIM: 192225
• UniProt ID: P19320
• Chromosome Location: 1p32-p31
• Pathway: Adaptive Immune System, organism-specific biosystem; African trypanosomiasis, organism-specific biosystem; African trypanosomiasis, conserved biosystem; Cell adhesion molecules (CAMs), organism-specific biosystem; Cell adhesion molecules (CAMs), conserved biosystem; Cellular roles of Anthrax toxin, organism-specific biosystem; Cytokine Signaling in Immune system, organism-specific biosystem
• Function: cell adhesion molecule binding; cell adhesion molecule binding; integrin binding