Recombinant Human VEGFA Protein, His-tagged

Cat.No.: VEGFA-407H
Product Overview: Human VEGF121 Protein, His Tag (Human VEGF121, His Tag) Ala 27 - Arg 147 (Accession # P15692-9) was produced in human 293 cells (HEK293).
Source: HEK293
Species: Human
Tag: His
Predicted N Terminal: His
Form: Lyophilized from sterile PBS, pH7.4, 10% Trehalose.
Bio-activity: Measured by its binding ability in a functional ELISA. Immobilized VEGFR2/R3-Fc at 1 μg/mL can bind Human VEGF121, His Tag with a linear range of 0.2-6.2 ng/mL.
Molecular Mass: Human VEGF121, His Tag is fused with a polyhistidine tag at the N-terminus, and has a calculated MW of 15 kDa. As a result of glycosylation, the reducing protein migrates as 17 kDa and 20 kDa, and the non-reducing protein migrates as 35-45 kDa in SDS-PAGE.
Endotoxin: Less than 1.0 EU per μg by the LAL method.
Purity: >95% as determined by reduced SDS-PAGE.
Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
Storage: Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.
Gene Name: VEGFA vascular endothelial growth factor A [ Homo sapiens ]
Official Symbol: VEGFA
Synonyms: VEGFA; vascular endothelial growth factor A; vascular endothelial growth factor , VEGF; VEGF A; VPF; vascular permeability factor; VEGF; MVCD1; MGC70609;
Gene ID: 7422
mRNA Refseq: NM_001025366
Protein Refseq: NP_001020537
MIM:
UniProt ID: P15692
Chromosome Location: 6p12
Pathway: Bladder cancer, organism-specific biosystem; Bladder cancer, conserved biosystem; Cytokine-cytokine receptor interaction, organism-specific biosystem; Cytokine-cytokine receptor interaction, conserved biosystem; Endochondral Ossification, organism-specific biosystem; Focal adhesion, organism-specific biosystem; Focal adhesion, conserved biosystem;
Function: cell surface binding; chemoattractant activity; cytokine activity; cytokine activity; extracellular matrix binding; fibronectin binding; growth factor activity; growth factor activity; heparin binding; heparin binding; platelet-derived growth factor receptor binding; protein binding; protein heterodimerization activity; protein homodimerization activity; receptor agonist activity; vascular endothelial growth factor receptor 1 binding; vascular endothelial growth factor receptor 2 binding; vascular endothelial growth factor receptor binding;

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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