Recombinant Human WNT3A(Ser19-Lys352) was expressed in HEK293.
Protein Wnt-3a is a protein that is encoded by the WNT3A gene. The WNT gene family consists of structurally related genes that encode secreted signaling proteins. These proteins have been implicated in oncogenesis, adipogenesis, etc. and in several other developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. Human Wnt3a shows 96% amino acid identity to mouse Wnt3a protein.
20 mM Tris-HCl, pH 7.2-7.4, 1% CHAPS, 0.1% BSA.
Predicted M.W.: 37 kDa;Actual M.W.: 41 kDa evaluated by SDS-PAGE under reducing conditions.
~ 75 % evaluated by SDS-PAGE under reducing conditions
This preparation can be stored at 4 centigrade for 6 months; Storage under frozen conditions may decrease activity; avoid freeze-thaw.To make stock solution of this protein, use Wnt Dilution Buffer provided (Tris-HCl buffer pH 7.2-7.4, 1% CHAPS, 0.1% BSA). To treat cells, dilute the stock solution at least 50 times in medium and use Wnt Dilution Buffer as a control.