Recombinant HIV-1 Nef gene cDNA (206 aa, derived from NL4-3 strain) fused with T7-His-TEV cleavage site Tag at N-terminal was expressed in E. coli.
Human immunodeficiency virus 1
0.5 mg/ml, sterile-filtered, in 20 mM pH 8.0 Tris-HCl Buffer, with proprietary formulation of NaCl, KCl, EDTA, arginine, DTT and Sucrose.
MASMTGGQQMGRGHHHHHHENLYFQGGFGGKWSKSSVIGWPAVRERMRRAEPAADGVGAVSRDLEKHGAITSSNT AANNAACAWLEAQEEEEVGFPVTPQVPLRPMTYKAAVDLSHFLKEKGGLEGLIHSQRRQDILDLWIYHTQGYFPD WQNYTPGPGVRYPLTFGWCYKLVPVEPDKVEEANKGENTSLLHPVSLHGMDDPEREVLEWRFDSRLAFHHVAREL HPEYFKNC
Keep at -80 centigrade for long term storage. Product is stable at 4 centigrade for at least 7 days.