Active Recombinant Influenza A H1N1(A/Puerto Rico/8/1934) NA protein

• Cat.No.: NA-178V
• Product Overview: Active Recombinant Influenza A H1N1(A/Puerto Rico/8/1934) NA protein(P03468.2) (Met1-Lys454) was expressed, the cell lysates are collected, and bio-activity was tested.

Specification

• Source: HEK293
• Species: H1N1
• Tag: N/A
• Form: Lyophilized from sterile 50 mM Tris, pH 8.0.
  Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its ability to cleave a fluorogenic substrate, 2'-(4-Methylumbelliferyl)-α-D-N-acetylneuraminic acid. The specific activity is > 150 U.
  One unit is defined as the amount of enzyme required to cleave 1 nmole of 2'-(4-Methylumbelliferyl)-α-D-N-acetylneuraminic acid per minute at pH 7.5 at 37°C.
• Molecular Mass: The recombinant influenza A H1N1 Neuraminidase comprises 454 amino acids and has a predicted molecular mass of 50.1 kDa.
• Protein Length: Met1-Lys454
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.