Recombinant spike protein fragment (Human betacoronavirus 2c EMC/2012)(AFS88936.1) (Glu367-Tyr606) was fused with the Fc region of rabbit IgG at the C-terminus.
Predicted N Terminal:
Lyophilized from sterile 100 mM Glycine, 10 mM NaCl, pH 7.2, 5%~8% trehalose and mannitol.
The recombinant spike protein fragment (Human betacoronavirus 2c EMC/2012) comprises 46.4 amino acids and has a predicted molecular mass of 51.5 kDa. It migrates as an approximately 54.3 kDa band in SDS-PAGE under reducing conditions.
< 1.0 EU per μg of the protein as determined by the LAL method.
>80 % as determined by SDS-PAGE.
Samples are stable for up to twelve months from date of receipt at -70ºC.
Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.