Recombinant Mouse Areg(Ser94-Lys191) was expressed in E. coli.
Predicted N Terminal:
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells. Marquardt, H. et al. (1984) Science 223:1079. The ED50 for this effect is typically 5-20 ng/mL.
Predicted Molecular Mass11.3 kDa
<0.10 EU per 1 μg of the protein by the LAL method.
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70 centigrade as supplied.1 month, 2 to 8 centigrade under sterile conditions after reconstitution.3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.