Recombinant Mouse Areg(Ser94-Lys191) was expressed in E. coli.
0.22 µm filtered protein solution is in PBS, pH 7.2.
ED50 = 0.04 - 0.2 µg/ml, corresponding to a specific activity of 0.5 - 2.5 x 10^4 units/mg, as determined by induction of BALB/3T3 cell proliferation.
The 98 amino acid recombinant protein has a predicted molecular mass of approximately 11.3 kD. The DTT-reduced and non-reduced protein migrate at approximately 16 kD and by SDS-PAGE. The N-terminal amino acid is Ser.
>95%, as determined by Coomassie stained SDS-PAGE.
Unopened vial can be stored between 2 centigrade and 8 centigrade for three months, at -20 centigrade for six months, or at -70 centigrade for one year. For maximum results, quick spin vial prior to opening. Stock solutions should be prepared at no less than 10 µg/mL in sterile buffer (PBS, HPBS, DPBS, and EBSS) containing carrier protein such as 1% BSA or HSA. After dilution, the cytokine can be stored between 2 centigrade and 8 centigrade for one month or from -20 centigrade to -70 centigrade for up to 3 months. Avoid repeated freeze/thaw cycles.