CD3D & CD3E-1251M
Recombinant extracellular domain (Met 1-Ala 105) of mouse CD3D (P04235) was fused with a flag tag at the C-terminus, constructed the plasmid 1; Recombinant extracellular domain (Met 1-Asp 108) of mouse CD3E (P22646) was fused with a polyhistidine tag at t
Predicted N Terminal:
Phe 22 & Asp 22
Lyophilized from sterile PBS, pH 7.4, 5%~8% trehalose and mannitol.
The recombinant heterodimer of mouse CD3D/CD3E comprises 254 (126+128) amino acids and has a calculated molecular mass of 29.2 (14.3+14.9) KDa. As a result of glycosylation, the apparent molecular mass of mouse CD3D/CD3E heterodimer is approximately 20 and 22 KDa in SDS-PAGE under reducing conditions.
< 1.0 EU per μg of the protein as determined by the LAL method.
(42.3+45.3) % as determined by SDS-PAGE.
Samples are stable for up to twelve months from date of receipt at -70ºC.
Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.