Active Recombinant Mouse CNTF Protein, His-tagged

• Cat.No.: CNTF-995M
• Product Overview: Recombinant mouse CNTF (NP_740756.1) (Ala2-Met198) was expressed with a polyhistidine tag at the N-terminus.

Specification

• Source: E. coli
• Species: Mouse
• Tag: His
• Predicted N Terminal: His
• Form: Lyophilized from sterile PBS, pH 7.4, 5%~8% trehalose and mannitol.
• Bio-activity: 1. Measured by its binding ability in a functional ELISA. Immobilized mouse CNTF at 10 μg/ml (100 μl/well) can bind biotinylated rat CNTFR-His. The EC50 of biotinylated rat CNTFR-His is 5-12 ng/ml.2. Measured in a cell proliferation assay using TF-1 human
• Molecular Mass: The recombinant mouse CNTF consists of 215 amino acids and predicts a molecular mass of 24.7 KDa. It migrates as an approximately 25 KDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: >95 % as determined by SDS-PAGE.
• Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
• Storage: Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: Cntf ciliary neurotrophic factor [ Mus musculus ]
• Official Symbol: CNTF
• Synonyms: CNTF; ciliary neurotrophic factor; AI429687; MGC41235
• Gene ID: 12803
• mRNA Refseq: NM_170786
• Protein Refseq: NP_740756
• Pathway: Cytokine-cytokine receptor interaction, organism-specific biosystem; Cytokine-cytokine receptor interaction, conserved biosystem; Delta-Notch Signaling Pathway, organism-specific biosystem; Jak-STAT signaling pathway, organism-specific biosystem; Jak-STAT
• Function: ciliary neurotrophic factor receptor binding; cytokine activity; growth factor activity; interleukin-6 receptor binding