Recombinant mouse CNTF (NP_740756.1) (Ala2-Met198) was expressed with a polyhistidine tag at the N-terminus.
Predicted N Terminal:
Lyophilized from sterile PBS, pH 7.4, 5%~8% trehalose and mannitol.
1. Measured by its binding ability in a functional ELISA. Immobilized mouse CNTF at 10 μg/ml (100 μl/well) can bind biotinylated rat CNTFR-His. The EC50 of biotinylated rat CNTFR-His is 5-12 ng/ml.2. Measured in a cell proliferation assay using TF-1 human
The recombinant mouse CNTF consists of 215 amino acids and predicts a molecular mass of 24.7 KDa. It migrates as an approximately 25 KDa band in SDS-PAGE under reducing conditions.
< 1.0 EU per μg of the protein as determined by the LAL method.
>95 % as determined by SDS-PAGE.
Samples are stable for up to twelve months from date of receipt at -70ºC.
Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.