Active Recombinant Mouse CPA2 Protein, His-tagged

• Cat.No.: CPA2-913M
• Product Overview: Recombinant mouse CPA2 (Q504N0) (Met 1-Tyr 417) was expressed, with a C-terminal polyhistidine tag.

Specification

• Source: HEK293
• Species: Mouse
• Tag: His
• Predicted N Terminal: Gln 17
• Form: Lyophilized from sterile PBS, pH 7.4, 5%~8% trehalose and mannitol.
• Bio-activity: Measured by its ability to cleave the colorimetric peptide substrate Ac-Phe-Thiaphe-OH in the presence of DTNB. The specific activity is >4000 pmoles/min/μg.
• Molecular Mass: The secreted recombinant mouse CPA2 (pro form) comprises 412 amino acids and has a calculated molecular mass of 46.6 kDa. It migrates as an approximately 47 kDa in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: >92 % as determined by SDS-PAGE.
• Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
• Storage: Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: Cpa2 carboxypeptidase A2, pancreatic [ Mus musculus ]
• Official Symbol: CPA2
• Synonyms: CPA2; carboxypeptidase A2, pancreatic; carboxypeptidase A2; MGC107514
• Gene ID: 232680
• mRNA Refseq: NM_001024698
• Protein Refseq: NP_001019869
• Pathway: Pancreatic secretion, organism-specific biosystem; Pancreatic secretion, conserved biosystem; Protein digestion and absorption, organism-specific biosystem; Protein digestion and absorption, conserved biosystem
• Function: carboxypeptidase activity; hydrolase activity; metal ion binding; metallocarboxypeptidase activity; metallopeptidase activity; peptidase activity; zinc ion binding