Recombinant extracellular domain of mouse Car14 (NP_035927.1) (Met 1-Met 290) was expressed with a C-terminal polyhistidine tag.
Predicted N Terminal:
Lyophilized from sterile 25mM Tris, 0.15mM NaCl, pH 7.51, 5%~8% trehalose and mannitol.
Measured by its esterase activity. The specific activity is >400 pmoles/min/μg, as measured with 1 mM 4-Nitrophenyl acetate and 0.8 μg enzyme at 400 nm in 100 μL of 12.5 mM Tris, 75 mM NaCl, pH 7.5.
The recombinant mouse CA14 consists of 286 amino acids and has a calculated molecular mass of 32.2 kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 45-48 kDa protein in SDS-PAGE under reducing conditions.
< 1.0 EU per μg of the protein as determined by the LAL method.
>95 % as determined by SDS-PAGE.
Samples are stable for up to twelve months from date of receipt at -70ºC.
Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.