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Home > Products > Recombinant Proteins > Recombinant Mouse Cd200r4 protein, His-tagged

Recombinant Mouse Cd200r4 protein, His-tagged

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By Species
Mouse(10)
By Source
Human cells(4)
N/A(1)
NS0(1)
By Tag
Fc(1)
His(2)
Cat.No.:
Cd200r4-250H
Product Overview:
Recombinant Mouse Cd200r4(Thr26-Thr241) fused with His tag at C-terminal was expressed in NS0.
Description:
CD200 R4, also known as CD200RLa, is a transmembrane protein in the immunoglobulin superfamily. Mature mouse CD200 R4 consists of a 216 amino acid (aa) extracellular domain (ECD), a 21 aa transmembrane segment, and an 8 aa cytoplasmic domain. The ECD contains one Ig-like V-type domain and one Ig-like C2-type domain. A positively charged lysine in the transmembrane likely mediates association with the signaling molecule DAP12. Within the ECD, mouse CD200 R4 shares 48% and 68% aa sequence identity with human and rat CD200 R4, respectively. CD200 R4 is expressed on monocytes, bone marrow derived macrophages and dendritic cells, NK cells, and resting mast cells. It is down-regulated following Fc epsilon RI-mediated mast cell activation. According to conflicting reports, CD200 R4 may or may not interact with CD200. R&D Systems in-house testing indicates that this recombinant mouse CD200 R4 binds to recombinant mouse CD200-Fc.
Source:
NS0
Species:
Mouse
Tag :
His
Predicted N Terminal:
Thr26
Form:
Lyophilized from a 0.2 μm filtered solution in PBS.
Bio-activity:
Measured by its binding ability in a functional ELISA. When Recombinant Mouse CD200 R4 is present at 0.5 μg/mL, Recombinant Mouse CD200 Fc Chimera binds with a typical ED50 of 0.8-4 μg/mL.
Molecular Mass:
Predicted Molecular Mass: 24 kDa
SDS-PAGE: 49-74 kDa, reducing conditions
Endotoxin:
<0.1 EU per 1 μg of the protein by the LAL method.
Purity:
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution:
Reconstitute at 500 μg/mL in PBS.


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Note: There will be extra charge for optional service!
Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-097-1828
FAX: 1-631-938-8127
EMAIL: info@creative-biomart.com

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