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Active Recombinant Mouse Cd274 protein(Met1-His239), hFc-tagged

Cat.No. : Cd274-821M
Product Overview : Recombinant Mouse Cd274 (NP_068693.1) (Met1-His239) was expressed in HEK293 with the Fc region of Human IgG1 at the C-terminus.
  • Specification
  • Gene Information
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Source : HEK293
Species : Mouse
Tag : C-hFc
Protein length : Met1-His239
Form : Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Bio-activity : 1. Measured by its binding ability in a functional ELISA.2.Immobilized human PD1-His at 10μg/mL (100μL/well) can bind mouse CD274-Fc, the EC50 of mouse CD274-Fc is 0.1-0.6μg/mL.
Molecular Mass : The recombinant mouse Cd274 consists 459 amino acids and predicts a molecular mass of 51.6 kDa. As a result of glycosylation, it migRates as an approximately 74.3 kDa band in SDS-PAGE under reducing conditions.
Endotoxin : < 1.0 EU per μg protein as determined by the LAL method.
Purity : > 95 % as determined by SDS-PAGE
Storage : Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
Gene Name : Cd274 CD274 antigen [ Mus musculus ]
Official Symbol : Cd274
Synonyms : CD274; CD274 antigen; programmed cell death 1 ligand 1; B7 homolog 1; PDCD1 ligand 1; programmed death ligand 1; B7h1; Pdl1; Pdcd1l1; Pdcd1lg1; A530045L16Rik;
Gene ID : 60533
mRNA Refseq : NM_021893
Protein Refseq : NP_068693

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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