Recombinant mouse CD55, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
CD55, also known as Complement decay-accelerating factor, GPI-anchored, is a member of the RCA(regulators of complement activation) family characterized by four to 30 SCRs(short consensus repeats) in their plasma-exposed regions. It is physiologically acting as an inhibitor of the complement system, but is also broadly expressed in malignant tumours.
Liquid. In Phosphate Buffered Saline (pH 7.4) containing 10% glycerol.
36.8kDa (336aa)40-57kDa (SDS-PAGE under reducing conditions)
DCGPPPDIPN ARPILGRHSK FAEQSKVAYS CNNGFKQVPD KSNIVVCLEN GQWSSHETFC EKSCVAPERL SFASLKKEYL NMNFFPVGTIVEYECRPGFR KQPPLPGKAT CLEDLVWSPV AQFCKKKSCP NPKDLDNGHI NIPTGILFGS EINFSCNPGY RLVGVSSTFC SVTGNTVDWDDEFPVCTEIH CPEPPKINNG IMRGESDSYT YSQVVTYSCD KGFILVGNAS IYCTVSKSDV GQWSSPPPRC IEKSKVPTKK PTINVPSTGTPSTPQKPTTE SVPNPGDQPT PQKPSTVKVS ATQHVPVTKT TVRHPIRTST DKGEPNTGLE HHHHHH
< 1.0 EU per 1 microgram of protein (determined by LAL method)
>95% by SDS - PAGE
Can be stored at +4centigrade short term (1-2 weeks). For long term storage, aliquot and store at -20Centigrade or -70Centigrade. Avoid repeated freezing and thawing cycles.
0.5mg/ml (determined by Absorbance at 280nm)