Recombinant Mouse Col1a1 fused with His tag was expressed in HEK293.
This gene encodes the alpha-1 subunit of the fibril-forming type I collagen, the most abundant protein of bone, skin and tendon extracellular matrices. The encoded protein, in association with alpha-2 subunit, forms heterotrimeric type I procollagen that undergoes proteolytic processing during fibril formation. Mice lacking the encoded protein die in utero caused by the rupture of a major blood vessel. Transgenic mice expressing significantly lower levels of this gene exhibit morphological and functional defects in mineralized and non-mineralized connective tissue and, progressive loss of hearing.
Tris-based buffer, 50% glycerol
Repeated freezing and thawing is not recommended. Store working aliquots at 4 centigrade for up to one week.
Store at -20 centigrade, for extended storage, conserve at -20 centigrade or -80 centigrade.