Recombinant mouse Creld2 (NP_083996.1) (Met1-Leu350) was expressed with the Fc region of human IgG1 at the C-terminus.
Predicted N Terminal:
Lyophilized from sterile PBS, pH 7.4, 5%~8% trehalose and mannitol.
Measured by its ability to induce adhesion of ATDC5 mouse chondrogenic cells.When cells are added to mCRELD2-Fc/His coated plates (0.625μg/mL, 100μL/well), approximately >30% will adhere specifically after 60 minutes at 37°C.
The recombinant mouse Creld2 consists 566 amino acids and predicts a molecular mass of 62.8 kDa.
< 1.0 EU per μg of the protein as determined by the LAL method.
>90 % as determined by SDS-PAGE.
Samples are stable for up to twelve months from date of receipt at -70ºC.
Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.