Recombinant mouse CTSZ, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
CTSZ, also known as cathepsin Z/X, is a relatively new cysteine protease of the papain family. Compared to other members of the papain family, CTSZ has a short proregion and unique insertions. Acting as a carboxypeptidase, CTSZ displays a unique specificity.
Liquid. In Phosphate Buffered Saline (pH 7.4) containing 10% glycerol.
32.8kDa (292aa)28-40KDa (SDS–PAGE under reducing conditions)
ARARLYFRSG QTCYHPIRGD QLALLGRRTY PRPHEYLSPA DLPKNWDWRN VNGVNYASVT RNQHIPQYCG SCWAHGSTSA MADRINIKRKGAWPSILLSV QNVIDCGNAG SCEGGNDLPV WEYAHKHGIP DETCNNYQAK DQDCDKFNQC GTCTEFKECH TIQNYTLWRV GDYGSLSGREKMMAEIYANG PISCGIMATE MMSNYTGGIY AEHQDQAVIN HIISVAGWGV SNDGIEYWIV RNSWGEPWGE KGWMRIVTST YKGGTGDSYNLAIESACTFG DPIVLEHHHH HH
< 1.0 EU per 1 microgram of protein (determined by LAL method)
>95% by SDS – PAGE
Can be stored at +4 centigrade short term (1-2 weeks). For long term storage, aliquot and store at -20 centigrade or -70 centigrade. Avoid repeated freezing and thawing cycles.
0.5mg/ml (determined by Absorbance at 280nm)