Recombinant mouse DPP7 (Q9ET221) (Met1-Arg 506) was expressed, with a C-terminal polyhistidine tag.
Predicted N Terminal:
Lyophilized from sterile PBS, pH 7.4, 5%~8% trehalose and mannitol.
Measured by its ability to cleave the fluorogenic peptide substrate, Lys-Pro-AMC(KP-AMC). The specific activity is >20,000 pmoles/min/μg.
The recombinant mouse DPP7 consists of 484 amino acids and predicts a molecular mass of 53.7 KDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rmDPP7 is approximately 65 KDa as a result of glycosylation.
< 1.0 EU per μg of the protein as determined by the LAL method.
>92 % as determined by SDS-PAGE.
Samples are stable for up to twelve months from date of receipt at -70ºC.
Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.