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Active Recombinant Mouse Dkk2 protein, His-tagged

Cat.No. : Dkk2-193M
Product Overview : Recombinant Mouse Dkk2(Ser26-Ile259) fused with Leu35Pro substitution and His tag at C-terminal was expressed in Insect cells.
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  • Gene Information
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Description : Dickkopf related protein 2 (Dkk-2) is a member of the Dickkopf family of secreted Wnt modulators. Dkk proteins contain a signal peptide and two conserved cysteine-rich domains that are separated by a linker region. The second cysteine-rich domain mediates Dkk-2 binding activities, and its interaction with LRP beta propellers has been mapped. The 226 aa, ~35 kDa mature mouse Dkk-2 shares 99%, 96%, 96%, 96% and 94% aa identity with rat, human, dog, horse and cow Dkk-2, respectively, and can activate the canonical Wnt signaling pathway in Xenopus embryos. Dkk proteins modify Wnt engagement of a receptor complex composed of a Frizzled protein and a low-density lipoprotein receptor-related protein, either LRP5 or LRP6. When LRP6 is over-expressed, direct high-affinity binding of Dkk-2 to LRP can enhance canonical Wnt signaling. However, when Dkk-2 and LRP6 form a ternary complex with Kremen2, Wnt signaling is inhibited due to internalization of Dkk-2/LRP6/Krm2 complexes. Thus, depending on the cellular context, Dkk-2 can either activate or inhibit canonical Wnt signaling. In contrast, binding of Dkk-1 or Dkk-4 to LRP is consistently antagonistic. Dkk proteins are expressed in mesenchymal tissues and control epithelial transformations. Dkk-2 expression has been studied most in bone and eye, although it is expressed as early as periimplantation in mice. Mouse Dkk-1 or Dkk-2 deficiencies have opposite effects on bone homeostasis, despite down-regulating Wnt antagonism in both cases. Dkk-2 expression is induced by Wnts in bone, and is thought to enhance bone density by promoting terminal differentiation of osteoblasts and mineral deposition. In contrast, Dkk-1 negatively regulates late osteoblast proliferation, which limits bone density. Dkk-2-deficient mice are blind, exhibiting faulty differentiation of corneal epithelium and ectopic blood vessels in the periocular mesenchyme.
Source : Insect cells
Species : Mouse
Tag : His
Predicted N Terminal : Ser26
Form : Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Bio-activity : Measured in a competitive binding assay. When Recombinant Mouse Kremen‑1 is immobilized at 4 µg/mL (100 µL/well), Recombinant Mouse Dkk-2 inhibits 50% binding of biotinylated Recombinant Human Dkk-1 (200 ng/mL) at the concentration range of 0.25-1.5 µg/mL.
Molecular Mass : Predicted Molecular Mass: 27 kDa
SDS-PAGE: 29-35 kDa, reducing conditions
Endotoxin : <0.1 EU per 1 μg of the protein by the LAL method.
Purity : >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Storage : Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution : Reconstitute at 100 μg/mL in PBS containing at least 0.1% human or bovine serum albumin.
Gene Name : Dkk2 dickkopf homolog 2 (Xenopus laevis) [ Mus musculus ]
Official Symbol : Dkk2
Synonyms : DKK2; dickkopf homolog 2 (Xenopus laevis); dickkopf-related protein 2; dkk-2; mDkk-2; dickkopf 2; dickkopf-2; dickkopf homolog 1;
Gene ID : 56811
mRNA Refseq : NM_020265
Protein Refseq : NP_064661

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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