Recombinant Mouse Dlk1 protein, His-tagged
Recombinant Mouse Dlk1(Ala24-Gln305) fused with His tag at C-terminal was expressed in NS0.
Predicted N Terminal:
Lyophilized from a 0.2 μm filtered solution in PBS.
Measured by its ability to inhibit adipocyte differentiation of mouse 3T3‑L1 mouse embryonic fibroblast adipose-like cells. Smas, C.M. et al. (1993) Cell 73:725. Recombinant Mouse Pref‑1/DLK1/FA1 at 2.5 μg/mL will inhibit adipocyte differentiation by more than 50%.
Predicted Molecular Mass: 31 kDa;SDS-PAGE: 49-66 kDa, reducing conditions.
<0.10 EU per 1 μg of the protein by the LAL method.
>95%, by SDS-PAGE with silver staining
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70 centigrade as supplied.1 month, 2 to 8 centigrade under sterile conditions after reconstitution.3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitute at 250 μg/mL in PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.