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Active Recombinant Mouse Epcam protein, Fc-tagged

Cat.No. : Epcam-191M
Product Overview : Recombinant Mouse Epcam(Gln24 & Arg81-Thr266) fused with Fc region of Mouse IgG2a at C-terminal was expressed in NS0.
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  • Gene Information
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Description : Epithelial Cellular Adhesion Molecule (EpCAM), also known as KS1/4, gp40, GA733-2, 17-1A, CD326 and TROP‑1, is a 40 kDa transmembrane glycoprotein composed of a 243 amino acid (aa) extracellular domain with two epidermal-growth-factor-like (EGF‑like) repeats, a 23 aa transmembrane domain, and a 26 aa cytoplasmic domain. Human and mouse EpCAM share 82% aa sequence identity. In mouse, EpCAM also shares 51% aa sequence homology with Trop‑2. EpCAM is detected in basolateral cell membranes of all simple epithelia and expression has been found to increase in epithelia tissues during fetal development. EpCAM has been shown to function as a homophilic Ca2+ independent adhesion molecule, but it does not structurally resemble any of the four major families of cell adhesion molecules. Homophilic adhesion via EpCAM requires the interaction of both EGF‑like repeats, with the first EGF‑like repeat mediating reciprocal interaction between EpCAM molecules on opposing cells, while the second repeat is involved in lateral interaction of EpCAM. EpCAM has been identified as a surface marker for pluripotent embryonic stem cells and is strongly associated with the maintenance of the undifferentiated state of ESCs. EpCAM is highly expressed on the surface of epithelial cancer cells including colon, stomach, lung, pancreas, prostate and breast.
Source : Mammalian cells
Species : Mouse
Tag : Fc
Predicted N Terminal : Arg81 detected. Gln24 inferred from enzymatic pyroglutamate treatment revealing Arg25
Form : Lyophilized from a 0.2 μm filtered solution in PBS.
Bio-activity : Measured by the ability of the immobilized protein to support the adhesion of the L Cells mouse fibroblast cell line. When 5 x 10^4 cells/well are added to Recombinant Mouse EpCAM/TROP-1 and Human Fibronectin coated plates, cell adhesion is enhanced in a dose dependent manner. The ED50 for this effect is typically 0.4-2.4 μg/mL.
Molecular Mass : Predicted Molecular Mass: 55 kDa
SDS-PAGE: 58-75 kDa and 8 kDa
Endotoxin : <0.1 EU per 1 μg of the protein by the LAL method.
Purity : >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Storage : Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution : Reconstitute at 500 μg/mL in PBS.
Gene Name : Epcam epithelial cell adhesion molecule [ Mus musculus ]
Official Symbol : Epcam
Synonyms : EPCAM; epithelial cell adhesion molecule; mEGP314; protein 289A; Trop-1 protein; lymphocyte antigen 74; epithelial glycoprotein 314; panepithelial glycoprotein 314; tumor-associated calcium signal transducer 1; EGP; Ly74; gp40; CD326; EGP-2; TROP1; Egp314; Ep-CAM; Tacsd1; GA733-2; Tacstd1;
Gene ID : 17075
mRNA Refseq : NM_008532
Protein Refseq : NP_032558
UniProt ID : Q99JW5

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (7)

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In which types of tissues is EpCAM predominantly expressed? 12/07/2022

EpCAM is highly expressed in epithelial tissues and some cancer cells.

How does EpCAM contribute to cell adhesion? 08/21/2022

It has a controversial role in cell adhesion, possibly contributing to both cell-cell adhesion and anti-adhesive properties.

What is the primary function of EpCAM in cells? 04/16/2022

EpCAM primarily functions in cell signaling, proliferation, and migration.

What role does EpCAM play in cancer progression? 03/19/2022

It often has a pro-tumorigenic role, promoting cancer cell proliferation, migration, and invasion.

How is EpCAM used as a biomarker in cancer diagnosis? 03/28/2021

EpCAM is used as a biomarker to detect and isolate circulating tumor cells.

What therapeutic strategies are being explored involving EpCAM? 11/23/2018

Strategies include developing EpCAM-targeted therapies for cancer treatment.

Are there known genetic alterations in EpCAM associated with specific health conditions? 11/07/2018

Genetic alterations in EpCAM are linked to some hereditary cancer syndromes.

Customer Reviews (3)

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Reviews
02/13/2022

    Reliable for assays, satisfied customer.

    09/09/2019

      High-quality product, consistent results.

      04/03/2019

        Quick delivery, product as described.

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