Active Recombinant Mouse F3 protein, His-tagged

• Cat.No.: F3-32M
• Product Overview: Recombinant Mouse F3(Ala29-Glu251) fused with His tag at C-terminal was expressed in NS0.

Specification

• Source: Mammalian cells
• Species: Mouse
• Tag: His
• Predicted N Terminal: Ala29
• Form: Lyophilized from a 0.2 μm filtered solution in Tris, NaCl and Brij-35.
• Bio-activity: Measured by its ability to activate Coagulation Factor VII in cleaving a fluorogenic peptide substrate Boc-VPR-AMC. The AC50 is <5 µg/mL.
• Molecular Mass: Predicted Molecular Mass: 26 kDa;
  SDS-PAGE: 38 kDa and 44 kDa, reducing conditions.
• Endotoxin: <1.0 EU per 1 μg of the protein by the LAL method.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Storage: Avoid repeated freeze-thaw cycles.
  6 months from date of receipt, -20 to -70 centigrade as supplied.
  3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
• Reconstitution: Reconstitute at 200 μg/mL in sterile 50 mM Tris, pH 9.0.

Gene Information

• Gene Name: F3 coagulation factor III [ Mus musculus ]
• Official Symbol: F3
• Synonyms: F3; coagulation factor III; tissue factor; TF; Cf3; Cf-3; CD142; AA409063
• Gene ID: 14066
• mRNA Refseq: NM_010171
• Protein Refseq: NP_034301
• UniProt ID: P20352
• Chromosome Location: 3 G1; 3 52.94 cM
• Pathway: Complement and Coagulation Cascades, organism-specific biosystem; Complement and coagulation cascades, organism-specific biosystem; Complement and coagulation cascades, conserved biosystem; Extrinsic Pathway, organism-specific biosystem; Formation of Fibrin Clot (Clotting Cascade), organism-specific biosystem; Hemostasis, organism-specific biosystem
• Function: cell surface binding; phospholipid binding; protease binding