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Home > Products > Recombinant Proteins > Recombinant Mouse Fcgrt/B2m protein, His/Strep II/Avi-tagged, Biotinylated

Recombinant Mouse Fcgrt/B2m protein, His/Strep II/Avi-tagged, Biotinylated

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Fcgrt & B2m-1546M
Product Name:
Recombinant Mouse Fcgrt/B2m protein, His/Strep II/Avi-tagged, Biotinylated
Product Overview:
Recombinant Mouse FCGRT(Ser 22 - Ser 297)/B2M(Ile 21 - Met 119), the subunit FCGRT / FcRN carries an Avi tag at the C-terminus, followed by a polyhistidine tag, the subunit Beta-2 microglobulin (B2M) carries a Twin Strep tag at the C-terminus was expressed in HEK293.
His/Strep II/Avi
Predicted N Terminal:
Ser 22 (FcRN) & Ile 21 (B2M)
Lyophilized from 0.22um filtered solution in PBS (pH 7.4) with Trehalose as protectant.
Molecular Mass:
The protein has a calculated MW of 34.7 kDa (FcRN), 14.7 kDa (B2M) . The protein migrates as 45-50 kDa (FCGRT), 15 kDa (B2M) respectively on a SDS-PAGE gel under reducing (R) condition due to glycosylation.
Protein length:
FCGRT(Ser 22 - Ser 297)/B2M(Ile 21 - Met 119)
Less than 1.0 EU per μg by the LAL method.
>95% as determined by SDS-PAGE.
Biotinylation of this product is performed using Avitag technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower. Please avoid repeated freeze-thaw cycles.No activity loss is observed after storage at:4-8 centigrade for 12 months in lyophilized state;-70 centigrade for 3 months under sterile conditions after reconstitution.
Reconstitute at 200 μg/mL in sterile deionized water. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the COA.

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Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127

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