Fcgrt & B2m-1545M
Recombinant Mouse FCGRT(Ser 22 - Ser 297)/B2M(Ile 21 - Met 119), subunit FCGRT / FcRN is fused with his-tag at the C-terminus and subunit Beta-2 microglobulin (B2M) is fused with Twin-Strep tag at the C-terminus was expressed in HEK293.
Predicted N Terminal:
Ser 22(FcRN) & Ile 21(B2M)
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization.
Has a calculated MW of 33 kDa (FCGRT / FcRN) and 14.7 kDa (B2M). The predicted N-terminus is Ser 22(FcRN) & Ile 21(B2M). DTT-reduced Protein migrates as 45-50 kDa (FCGRT) and 18 kDa (B2M) respectively due to glycosylation.
FCGRT(Ser 22 - Ser 297)/B2M(Ile 21 - Met 119)
Less than 1.0 EU per μg of the Mouse FcRn / FCGRT & B2M by the LAL method.
>95% as determined by SDS-PAGE.
Avoid repeated freeze-thaw cycles.No activity loss was observed after storage at:In lyophilized state for 1 year (4 centigrade); After reconstitution under sterile conditions for 3 months (-70 centigrade).
See Certificate of Analysis for reconstitution instructions and specific concentrations.