Recombinant Mouse Fgfr3 Protein, His & Fc-tagged
Recombinant extracellular domain (Met 1-Tyr 367) of mouse FGFR3 (NP_032036.2) precursor was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus.
Predicted N Terminal:
Lyophilized from sterile PBS, pH 7.4, 5%~8% trehalose and mannitol.
Measured by its ability to bind mouse aFGF in a functional ELISA.
The recombinant mouse FGFR3/Fc is a disulfide-linked homodimer after removal of the signal peptide. The reduced monomer consists of 595 amino acids and has a predicted molecular mass of 66 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rm FGFR3/Fc monomer is approximately 100-110 kDa due to glycosylation.
< 1.0 EU per μg of the protein as determined by the LAL method.
>92 % as determined by SDS-PAGE.
Samples are stable for up to twelve months from date of receipt at -70ºC.
Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.