Recombinant Mouse Flt3l was expressed in E. coli.
FLT3 ligand is a receptor for the fl cytokine has a tyrosine-protein kinase activity & a growth factor that regulates proliferation of early hematopoietic cells. Flt3-Ligand synergizes with other CSFs and interleukins to induce growth and differentiation.
The FLT3L Mouse protein was lyophilized without any additives.
The ED50, calculated by the dose-dependent proliferation of mouse AML5 cells is 1.3-2.0ng/ml.
163 amino acids
MTPDCYFSHS PISSNFKVKF RELTDHLLKD YPVTVAVNLQ DEKHCKALWS LFLAQRWIEQ LKTVAGSKMQ TLLEDVNTEI HFVTSCTFQP LPECLRFVQT NISHLLKDTC TQLLALKPCI GKACQNFSRC LEVQCQPDSS TLLPPRSPIA LEATELPEPR PRQ.
Greater than 98.0% as determined by SDS-PAGE.
Lyophilized Flt3-Ligand although stable at room temperature for 3 weeks, should be stored desiccated below -18 centigrade. Upon reconstitution Flt-3 Ligand should be stored at 4 centigrade between 2-7 days and for future use below -18 centigrade.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
It is recommended to reconstitute the lyophilized Flt3-Ligand in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.