Active Recombinant Mouse Folr1, His-tagged

• Cat.No.: Folr1-4045M
• Product Overview: Mouse FOLR1 Protein (Mouse FOLR1, His Tag) Thr 25 - Ser 255 (Accession # NP_032060.2) was produced in human 293 cells (HEK293).

Specification

• Source: HEK293
• Species: Mouse
• Tag: His
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4, 10% Trehalose.
• Bio-activity: Measured in a competitive binding assay. When Folic Acid-Bovine Serum Albumin is immobilized at 5 μg/mL (100 μL/well), Mouse FOLR1, His Tag inhibits 50% binding of biotinylated rmFOLR1 (7.9 nM) at the concentration range of 10-100 nM.
• Molecular Mass: Mouse FOLR1, His Tag is fused with a polyhistidine tag at the C-terminus, and has a calculated MW of 27.5 kDa. The predicted N-terminus is Thr 25. The reducing (R) protein migrates as 45-50 kDa in SDS-PAGE due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Stability: Samples are stable for up to twelve months from date of receipt at -70 centigrade
• Storage: Store it under sterile conditions at -20 centigrade to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: Folr1 folate receptor 1 (adult) [ Mus musculus ]
• Official Symbol: Folr1
• Synonyms: FOLR1; folate receptor 1 (adult); folate receptor alpha; FR-alpha; folate binding protein 1; folate-binding protein 1; FBP1; Folbp1; Folbp-1
• Gene ID: 14275
• mRNA Refseq: NM_001252552
• Protein Refseq: NP_001239481
• Pathway: Endocytosis, organism-specific biosystem; Endocytosis, conserved biosystem
• Function: folic acid binding; folic acid transporter activity; receptor activity