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Home > Products > Recombinant Proteins > Recombinant Mouse Fzd10 protein, Fc-tagged

Recombinant Mouse Fzd10 protein, Fc-tagged

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His(4)
Cat.No.:
Fzd10-276M
Product Overview:
Recombinant Mouse Fzd10(Ile22-Phe166) fused with Fc region of Mouse IgG2a at C-terminal was expressed in NS0.
Description:
Frizzled-10, also known as CD350, is a 68 kDa seven pass transmembrane glycoprotein in the frizzled family of Wnt receptors. The N-terminal extracellular region of Frizzled-10 contains a cysteine-rich domain that comprises the Wnt binding domain and mediates receptor oligomerization. The C-terminal cytoplasmic tail contains a PDZ-interaction motif. Within the cysteine-rich domain, human Frizzled-10 shares 94% and 99% aa sequence identity with human and rat Frizzled-10, respectively. Frizzled-10 is expressed during embryogenesis in the primitive streak, dorsal neural tube, developing brain, limb bud, and airway epithelium. It is induced by Shh and colocalizes with Shh and Wnt-7a in the neural tube. In the adult, Frizzled-10 is expressed in placenta, gastric glands, and colon and renal tubule epithelial cells. Frizzled-10 associates with LRP5 and transduces Wnt-1, -7a, -7b, and -8 signals, resulting in the stabilization of cytoplasmic beta-Catenin. It is important for the differentiation of sensory neurons. Frizzled-10 is also up-regulated in some cancers and transformed cell lines. It binds hypoxia inducible gene 2, which promotes oncogenic Wnt signaling and functions as an autocrine growth factor for renal cell carcinomas.
Source:
NS0
Species:
Mouse
Tag :
Fc
Predicted N Terminal:
Ile22
Form:
Lyophilized from a 0.2 μm filtered solution in PBS.
Bio-activity:
Measured by its ability to bind biotinylated recombinant mouse Wnt-3a in a functional ELISA with an ED50 of 10-60 ng/mL.
Molecular Mass:
Predicted Molecular Mass: 44 kDa
SDS-PAGE: 53-61 kDa, reducing conditions
Endotoxin:
<0.1 EU per 1 μg of the protein by the LAL method.
Purity:
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution:
Reconstitute at 200 μg/mL in PBS.


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Note: There will be extra charge for optional service!
Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-097-1828
FAX: 1-631-938-8127
EMAIL: info@creative-biomart.com

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