Address: 45-1 Ramsey Road, Shirley, NY 11967, USA    USA: 1-631-559-9269  1-631-448-7888   Fax: 1-631-938-8127  Europe: 44-207-097-1828   Email: info@creative-biomart.com
Home > Products > Recombinant Proteins > Recombinant Mouse Gdf15 protein

Recombinant Mouse Gdf15 protein

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By Species
Human(14)
Mouse(6)
Rat(5)
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Fc(1)
His(8)
N/A(5)
Cat.No.:
Gdf15-393M
Product Overview:
Recombinant Mouse Gdf15(Ser189-Ala303) was expressed in E. coli.
Description:
Growth Differentiation Factor 15 (GDF-15), also called Macrophage Inhibitory Cytokine 1 (MIC-1), Placental Transforming Growth Factor beta, Prostate-derived Factor, and Placental Bone Morphogenetic Protein, is a divergent member of the TGF-beta superfamily. Cellular responses to TGF-beta proteins are mediated by hetero-oligomeric complexes of type I and type II serine/threonine kinase receptors. GDF-15 is highly expressed in placenta and brain, and it is expressed at lower levels in kidney, pancreas, prostate, and colon. Similar to other TGF-beta family proteins, GDF-15 is synthesized as a large precursor protein that is cleaved at a dibasic cleavage site (RxxR) to release the mature protein. Mature mouse GDF-15 shares 66% and 97% amino acid sequence identity with the human and rat proteins, respectively. The C-terminal domain of GDF-15 contains seven characteristic conserved cysteine residues necessary for the formation of the cysteine knot and the single inter-chain disulfide bond. Biologically active GDF-15 is a disulfide-linked homodimer of the mature protein. GDF-15 has been shown to have various functions, including inhibition of TNF-alpha production from lipopolysaccharide-stimulated macrophages and the induction of cartilage formation. GDF-15 also promotes neuronal survival, and hypothalamic expression of GDF-15 causes appetite suppression via modulation of neuropeptide Y and pro-opiomelanocortin levels. GDF-15 is cardioprotective via inhibition of platelet activation, limiting atherosclerosis, promoting recovery following myocardial infarction, and regulating angiogenesis. Exposure of cardiomyocytes to GDF-15 results in Smad2 and Smad3 phosphorylation.
Source:
E. coli
Species:
Mouse
Predicted N Terminal:
Ser189
Form:
Lyophilized from a 0.2 μm filtered solution in HCl.
Bio-activity:
Measured by its binding ability in a functional ELISA. When Recombinant Mouse GSF-15 is immobilized at 2 µg/mL (100 µL/well), the concentration of Recombinant Mouse Activin RIB/ALK-4 Fc Chimera that produces 50% of the optimal binding response is approximately 1-6 μg/mL.
Molecular Mass:
Predicted Molecular Mass: 13 kDa;
SDS-PAGE: 9-13 kDa, reducing conditions.
Protein length:
Ser189-Ala303
Endotoxin:
<0.10 EU per 1 μg of the protein by the LAL method.
Purity:
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution:
Reconstitute at 250 μg/mL in 4 mM HCl.
Shipping:
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.


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Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-097-1828
FAX: 1-631-938-8127
EMAIL: info@creative-biomart.com

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