Recombinant Mouse Gdf15 (His193~Val296) was expressed in E. coli with N-terminal his tag.
Lyophilized from 20mM Tris, 150mM NaCl,pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
The protein has a calculated MW of 18.2kDa. The protein migrates as 20kDa on a SDS-PAGE gel under reducing (R) condition due to glycosylation.
< 1.0EU per 1µg (determined by the LAL method)
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.