IL12A & IL12B-1245M
Recombinant Mouse IL12A & IL12B Protein, Fc & His-tagged
Recombinant p35 subunit of mouse IL12, termed as IL12A (P43431) (Met 1-Ala 215) was fused with the Fc region of human IgG1 at the C-terminus, constructed the plasmid 1; Recombinant p40 subunit of mouse IL12, termed as IL12B (P43432) (Met 1-Ser 335) was fu
Predicted N Terminal:
Arg 23 & Met 23
Lyophilized from sterile PBS, pH 7.4, 5%~8% trehalose and mannitol.
1. Measured by its binding ability in a functional ELISA. Immobilized mouse IL12RB2-His at 10 μg/ml (100 μl/well) can bind Mouse IL12A & IL12B Heterodimer Protein. The EC50 of Mouse IL12A & IL12B Heterodimer Protein is 50.2-117.2 ng/ml. 2. Measured in a c
The recombinant mouse IL12 heterodimer of IL12A/IL12B, IL12A is Fc chimera, comprises 758 (434 +324) amino acids and has a calculated molecular mass of 85.9 (48.7 + 37.2) kDa. The apparent molecular mass of rh IL12 heterodimer is approximately 55 & 45 kDa respectively in SDS-PAGE under reducing conditions.
< 1.0 EU per μg of the protein as determined by the LAL method.
>85 % as determined by SDS-PAGE.
Samples are stable for up to twelve months from date of receipt at -70ºC.
Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.