Active Recombinant Mouse Il10 protein
Cat.No. : | Il10-214M |
Product Overview : | Recombinant Mouse Il10(Ser19-Asn178) was expressed in E. coli. |
- Specification
- Gene Information
- Related Products
Description : | Interleukin 10, also known as cytokine synthesis inhibitory factor (CSIF), is the charter member of the IL-10 family of alpha -helical cytokines that also includes IL‑19, IL‑20, IL-22, and IL-24. IL-10 is secreted by many activated hematopoietic cell types as well as hepatic stellate cells, keratinocytes, and placental cytotrophoblasts. Mature rat IL‑10 shares 85% amino acid sequence identity with mouse and 71%-79% amino acid sequence identity with bovine, canine, equine, feline, human, ovine, and porcine IL-10. Whereas human IL-10 is active on mouse cells, mouse IL-10 does not act on human cells. IL-10 is a 178 amino acid molecule that contains two intrachain disulfide bridges and is expressed as a 36 kDa noncovalently associated homodimer. The IL-10 dimer binds to two IL‑10 R alpha /IL‑10 R1 chains, resulting in recruitment of two IL-10 R beta /IL-10R2 chains and activation of a signaling cascade involving JAK1, TYK2, and STAT3. IL‑10 R beta does not bind IL-10 by itself but is required for signal transduction. IL-10 R beta also associates with IL-20 R alpha, IL-22 R alpha, or IL-28 R alpha to form the receptor complexes for IL-22, IL-26, IL-28, and IL-29. IL-10 is a critical molecule in the control of viral infections and allergic and autoimmune inflammation. It promotes phagocytic uptake and Th2 responses but suppresses antigen presentation and Th1 proinflammatory responses. |
Source : | E. coli |
Species : | Mouse |
Predicted N Terminal : | Ser19 |
Form : | Lyophilized from a 0.2 μm filtered solution in PBS. |
Bio-activity : | Measured in a cell proliferation assay using MC/9‑2 mouse mast cells. Thompson-Snipes, L. et al. (1991) J. Exp. Med. 173:507. The ED50 for this effect is 0.12-0.72 ng/mL. |
Molecular Mass : | Predicted Molecular Mass: 19 (monomer) kDa SDS-PAGE: 16 kDa, reducing conditions |
Endotoxin : | <0.1 EU per 1 μg of the protein by the LAL method. |
Purity : | >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining. |
Storage : | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 centigrade as supplied. 1 month, 2 to 8 centigrade under sterile conditions after reconstitution. 3 months, -20 to -70 centigrade under sterile conditions after reconstitution. |
Reconstitution : | Reconstitute at 200 μg/mL in PBS. |
Gene Name : | Il10 interleukin 10 [ Mus musculus ] |
Official Symbol : | Il10 |
Synonyms : | IL10; interleukin 10; interleukin-10; cytokine synthesis inhibitory factor; CSIF; Il-10; |
Gene ID : | 16153 |
mRNA Refseq : | NM_010548 |
Protein Refseq : | NP_034678 |
UniProt ID : | P18893 |
Products Types
◆ Recombinant Protein | ||
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Il10-082M | Active Recombinant Mouse Il10 Protein | +Inquiry |
Il10-006I | Active Recombinant Rat Il10 Protein (160 aa) | +Inquiry |
◆ Lysates | ||
IL10-2079HCL | Recombinant Human IL10 cell lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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