Recombinant mouse IMPAD1, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
IMPAD1, also known as inositol monophosphatase 3, may play a role in the formation of skeletal elements derived through endochondral ossification, possibly by clearing adenosine 3, 5-bisphosphate produced by Golgi sulfotransferases during glycosaminoglycan sulfation.
Liquid. In Phosphate Buffered Saline (pH 7.4) containing 10% glycerol.
36.2kDa (332aa)28-40KDa (SDS-PAGE under non-reducing conditions.)
ADPGRFSLFG LGSEPAAGEA EVASDGGTVD LREMLAVAVL AAERGGDEVR RVRESNVLHE KSKGKTREGA DDKMTSGDVL SNRKMFYLLKTAFPNVQINT EEHVDASDKE VIVWNRKIPE DILKEIAAPK EVPAESVTVW IDPLDATQEY TEDLRKYVTT MVCVAVNGKP VLGVIHKPFSEYTAWAMVDG GSNVKARSSY NEKTPKIIVS RSHAGMVKQV ALQTFGNQTS IIPAGGAGYK VLALLDVPDM TQEKADLYIH VTYIKKWDICAGNAILKALG GHMTTLNGEE ISYTGSDGIE GGLLASIRMN HQALVRKLPD LEKSGHHHHH HH
< 1.0 EU per 1 microgram of protein (determined by LAL method)
>95% by SDS - PAGE
Can be stored at +4centigrade short term (1-2 weeks). For long term storage, aliquot and store at -20Centigrade or -70Centigrade. Avoid repeated freezing and thawing cycles.
0.5mg/ml (determined by Absorbance at 280nm)