Recombinant mouse LGMN, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
LGMN, also known as legumain, is a lysosomal cysteine protease found in all mouse tissues, but was particularly abundant in kidney and placenta. It plays a pivotal role in the endosomal/lysosomal degradation system because the Legumain deficiency causes the accumulation of pro cathepsins B, H and L, another group of lysosomal cysteine proteases. Over expression of Legumain in tumors is significant for invasion/metastasis.
Liquid. In Phosphate Buffered Saline (pH 7.4) containing 10% glycerol.
48.6kDa (426aa)40-57KDa (SDS–PAGE under reducing conditions)
VPVGVDDPED GGKHWVVIVA GSNGWYNYRH QADACHAYQI IHRNGIPDEQ IIVMMYDDIA NSEENPTPGV VINRPNGTDV YKGVLKDYTGEDVTPENFLA VLRGDAEAVK GKGSGKVLKS GPRDHVFIYF TDHGATGILV FPNDDLHVKD LNKTIRYMYE HKMYQKMVFY IEACESGSMMNHLPDDINVY ATTAANPKES SYACYYDEER GTYLGDWYSV NWMEDSDVED LTKETLHKQY HLVKSHTNTS HVMQYGNKSI STMKVMQFQGMKHRASSPIS LPPVTHLDLT PSPDVPLTIL KRKLLRTNDV KESQNLIGQI QQFLDARHVI EKSVHKIVSL LAGFGETAER HLSERTMLTAHDCYQEAVTH FRTHCFNWHS VTYEHALRYL YVLANLCEAP YPIDRIEMAM DKVCLSHYLE HHHHHH
< 1.0 EU per 1 microgram of protein (determined by LAL method)
>90% by SDS – PAGE
Can be stored at +4 centigrade short term (1-2 weeks). For long term storage, aliquot and store at -20 centigrade or -70 centigrade. Avoid repeated freezing and thawing cycles.
0.5mg/ml (determined by Absorbance at 280nm)