Recombinant Mouse Lag3(Gly 24 - Leu 442) fused with His tag at the C-terminus was expressed in HEK293.
Predicted N Terminal:
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization.
Has a calculated MW of 47.1 kDa. The reducing (R) protein migrates as 55-65 kDa in SDS-PAGE due to glycosylation.
Gly 24 - Leu 442
Less than 1.0 EU per μg of the Mouse LAG-3, His Tag by the LAL method.
>95% as determined by SDS-PAGE.
Avoid repeated freeze-thaw cycles.No activity loss was observed after storage at:In lyophilized state for 1 year (4 centigrade); After reconstitution under sterile conditions for 3 months (-70 centigrade).